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Production of biosurfactant by Pseudomonas aeruginosa grown on cashew apple juice
Authors:Rocha  Maria V. P.  Souza  Maria C. M.  Benedicto  Sofia C. L.  Bezerra  Márcio S.  Macedo  Gorete R.  Pinto  Gustavo A. Saavedra  Gonçalves  Luciana R. B.
Affiliation:Departmento de Engenharia Química, Campus do Pici, Universidade Federal do Ceará, Bloco 709, 60455-760, Fortaleza, CE, Brazil.
Abstract:In this work, the ability of biosurfactant production by Pseudomonas aeruginosa in batch cultivation using cashew apple juice (CAJ) and mineral media was evaluated. P. aeruginosa was cultivated in CAJ, which was supplemented with peptone (5.0 g/L) and nutritive broth. All fermentation assays were performed in Erlenmeyer flasks containing 300 mL, incubated at 30 degrees C and 150 rpm. Cell growth (biomass and cell density), pH, and superficial tension were monitored vs time. Surface tension was reduced by 10.58 and 41% when P. aeruginosa was cultivated in nutrient broth and CAJ supplemented with peptone, respectively. These results indicated that CAJ is an adequate medium for growth and biosurfactant production. Best results of biosurfactant production were obtained when CAJ was supplemented with peptone.
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