Signal amplification via intramolecular energy transfer using tripodal neutral iridium(III) complexes upon binding to avidin |
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| Authors: | Kwon Tae-Hyuk Kwon Jongchul Hong Jong-In |
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| Institution: | Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea. |
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| Abstract: | A neutral tripod system for biotin-avidin assays, which is composed of biotin, FIrpic as an energy acceptor, and mCP as an energy donor, offers remarkable sensitivity over traditional transition metal based protein probes due to the intramolecular energy transfer and increased hydrophobicity associated with the avidin binding site and neutral probe 1. |
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