Studies on the metabolism of gomisin A (TJN-101). II. Structure determination of biliary and urinary metabolites in rat |
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Authors: | Y Ikeya H Mitsuhashi H Sasaki Y Matsuzaki T Matsuzaki E Hosoya |
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Institution: | Research Institute for Biology & Chemistry, Tsumura & Co., Ibaraki, Japan. |
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Abstract: | After oral administration of gomisin A (1) to rats, the bile and urine were collected and treated with beta-glucuronidase and arylsulfatase. Seven metabolites, met B (2), met A-III (3), met E (4), met D (5), met F (6), met G (7), and met H (8) were isolated from the bile treated with the enzymes. Eight metabolites 2-8, and met A-II (9) were isolated from the urine treated with the enzymes. A major metabolite 2, and two minor metabolites 3 and 9 were identified as met B, met A-III, and met A-II, respectively, which are oxidative products of 1 formed by rat liver S9 mix. The structures of five new metabolites 4-7, and 8 were determined on the basis of chemical and spectral studies. |
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