A (1→4)-β-D-Glucuronan Excreted by a Mutant of the Rhizobium Meliloti M5N1 Strain |
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| Authors: | Josiane Courtois Jean-Paul Seguin Sophie Declomesnil Alain Heyraud Philippe Colin-Morel Luciana Dantas |
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| Institution: | 1. Laboratoire de Biotechnologie Microbienne , Université de Picardie Avenue des Facultés , Le Bailly, 80025, Amiens, Cedex 1, France;2. Laboratoire de Génie Cellulaire, Faculté des Sciences , Université de Picardie , 33 Rue Saint Leu, 80039, Amiens, Cedex, France;3. Centre de Recherches sur les Macromolécules Végétales (CNRS) , B.P. 53 X, 38041, Grenoble, Cedex, France |
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| Abstract: | Abstract A mutant of the R. meliloti M5N1 strain has been selected. This strain, R. meliloti M5N1 CS (NCIMB 40472), excretes an extracellular material composed of 2-O-Ac-β-GlcpA, 3-O-Ac-β-GlcpA, 2,3-di-O-Ac-β-GlcpA and three species of β-GlcpA residues 1→4 linked. For the culture conditions used, the weight average molecular weight of the polymer varied in the range of 6 × 104 < Mw < 4 × 105. High molecular weight glucuronate forms thermoreversible gels at 5 g L?l. In the presence of divalent cation such as Ca2+ or trivalent cations such as Cr3+ or Fe3 +, cross linking of the polymer occurs. This polysaccharide is the first exocellular (1→4)-β-D-glucuronan produced by a R. meliloti strain. |
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