Sortase-Mediated Transpeptidation for Site-Specific Modification of Peptides,Glycopeptides, and Proteins |
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Authors: | Zhimeng Wu Zhongwu Guo |
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Institution: | Department of Chemistry , Wayne State University , Detroit , MI , 48202 , USA |
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Abstract: | Sortases are a family of transpeptidases found in gram-positive bacteria responsible for covalent anchoring of cell surface proteins to bacterial cell walls. It has been discovered that sortase A (SrtA) of Staphylococcus aureus origin is rather promiscuous and can accept various molecules as substrates. As a result, SrtA has been widely used to ligate peptides and proteins with a variety of nucleophiles, and the ligation products are useful for research in chemical biology, proteomics, biomedicine, etc. This review summarizes the recent applications of SrtA with special emphasis on SrtA-catalyzed ligation of carbohydrates with peptides and proteins. |
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Keywords: | Sortase Enzymatic transpeptidation Peptide Protein Glycopeptide GPI anchor |
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