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Synthesis and MALDI-TOF MS Analysis of Protected Oligosaccharide Components of N-Glycoproteins |
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| Authors: | Károly Ágoston Gyöngyi Gyémánt László Kalmár János Kerékgyártó Zoltán Szurmai Boglárka Döncző |
| Affiliation: | 1. RCNS-HAS, Institute of Organic Chemistry H-1117, Budapest, Magyar Tudósok k?rútja 2, Hungaryagoston.karoly@ttk.mta.hu;3. Institute of Inorganic and Analytical Chemistry, Faculty of Sciences and Technology, University of Debrecen, Debrecen H-4032, Debrecen, Egyetem tér 1, Hungary;4. Horvath Laboratory of Bioseparation Sciences, University of Debrecen H-4032, Debrecen, Nagyerdei k?rút 98., Hungary;5. Bio-organic Laboratory, Department of Botany, Faculty of Sciences and Technology, University of Debrecen H-4032, Debrecen, Egyetem tér 1, Hungary;6. Bio-organic Laboratory, Department of Ecology, Faculty of Sciences and Technology, University of Debrecen H-4032, Debrecen Egyetem tér 1, Hungary |
| Abstract: | Essential components of N-glycoproteins were synthesized in octyl glycoside form starting from 3-O-allyl-D-glucose derivative. The β-mannosidic linkage was formed by the oxidation reduction method. MALDI-TOF mass spectrometry and its post-source decay (PSD) mode were used for identification and structural elucidation of protected synthetic oligosaccharides related to N-glycoproteins. Most fragments, identified in the PSD spectra, were products of the cleavage of glycosidic bonds. |
| Keywords: | Oligosaccharide synthesis β-Mannoside N-Glycoprotein MALDI-TOF MS Post-source decay (PSD) |