UCHP Method for Oligosaccharide Combinatorial Library Synthesis |
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| Authors: | Shiro Komba Sachiko Machida |
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| Affiliation: | 1. Biomolecular Engineering Laboratory , National Food Research Institute , 2-1-12 Kannondai, Tsukuba, Ibaraki, 305-8642, Japan;2. PRESTO, Japan Science and Technology Agency , 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan;3. Biomolecular Engineering Laboratory , National Food Research Institute , 2-1-12 Kannondai, Tsukuba, Ibaraki, 305-8642, Japan |
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| Abstract: | A new methodology for oligosaccharide combinatorial library synthesis using a special hydroxy protecting group, the uni-chemo hydroxy protection (UCHP) group, was developed. The UCHP group was composed of oligomeric amino acid derivatives. The amino terminals of UCHP groups were protected by either Boc or Fmoc groups. By using these two types of UCHP, five kinds of trigalactoses [Galβ1-3Galβ1-3Gal, Galβ1-3(Galβ1-4)Gal, Galβ1-4Galβ1-3Gal, Galβ1-3Galβ1-4Gal, and Galβ1-4Galβ1-4Gal] were successfully synthesized on a solid support as a model of oligosaccharide combinatorial library. Each step of all reactions was also successfully monitored using a combination of two colorimetric tests, chloranil and methyl red-DIC. |
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| Keywords: | UCHP Oligosaccharide combinatorial library Solid-phase synthesis |
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