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A New Extraction and Purification Methodology of Bowman-Birk Inhibitors from Seeds and Germinated Seeds of Macrotyloma axillare |
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| Authors: | C&# sar,Jorgino Julio,de Santana,Marcos Aur&# lio,de Oliveira,Marcelo E.,Santos,Alexandre G.,de Miranda,Alexandre A. Cerqueira,Santos, Alexandre M. Costa,Santoro, Marcelo Matos,de Andrade,Milton H. Guerra |
| Affiliation: | 1.Departamento de Ciências Biológicas, Núcleo de Pesquisas em Ciências Biológicas, ICEB–UFOP, Ouro Preto, Brazil ;2.Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, Brazil ;3.Departamento de Ciências Biológicas, UEFS, Salvador, Brazil ; |
| Abstract: | The Macrotyloma axillare Bowman-Birk inhibitors from seeds and germinated seeds (120 h) were purified by an alternative methodology and revealed the presence of different contents of inhibitors for each treatment. Mass analyses of inhibitors, from seeds and germinated seeds, showed masses of about 7 and 8 kDa. The 120 h-germinated seed fractions presented higher activity isoforms than seeds without treatment. |
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