Stability-Indicating LC Method for Assay of Cholecalciferol |
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Authors: | Desai Namita D Singh Pirthipal P Amin Purnima D Jain Satishkumar P |
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Institution: | 1.Pharmaceutical Sciences and Technology Division, University Institute of Chemical Technology (Autonomous), University of Mumbai, Matunga, Mumbai, 400019, India ; |
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Abstract: | This paper discusses the development of a stability-indicating reversed-phase LC method for analysis of cholecalciferol as the bulk drug and in formulations. The mobile phase was acetonitrile–methanol–water 50:50:2 (v/v). The calibration plot for the drug was linear in the range 0.4–10 μg mL−1. The method was accurate and precise with limits of detection and quantitation of 64 and 215 ng, respectively. Mean recovery was 100.71%. The method was used for analysis of cholecalciferol in pharmaceutical formulations in the presence of its degradation products and commonly used excipients. |
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