新型有机荧光染料嵌合的核壳荧光纳米材料的研制

采用油包水的反相微乳液方法,首次以羊抗人免疫球蛋白(IgG)标记的异硫氰酸荧光素(FITC)为核材料,成功地制备了FITC的核壳荧光纳米颗粒,克服了采用传统方法制备核壳荧光纳米颗粒中存在的荧光染料泄露的问题.制备的这种核壳荧光纳米颗粒比细胞小很多,且具有生物亲和性,可为纳米生物传感器件提供新型材料.基于该核壳荧光纳米颗粒的标记方法也为生物医学提供了一种新型的非同位素分析方法.

A Study of a Novel Organic Fluorescent Core-shell Nanoparticle

A novel fluorescent core-shell nanoparticle has been developed by encapsulating the fluorophore into silica shell with a reverse microemulsion technique. The FITC encapsulation is greatly enhanced by conjucting a water-soluble protein IgG which significantly reduces FITC leaching from the nanoparticles when immersed inside water. The FITC leaching problem of the nanoparticles prepared with IgG has been much less severe than that with the FITC nanoparticles prepared with unmodified dye or dye modified with dextrans of higher molecular weight. The fluorescent core-shell nanoparticles are also pH sensible. They can be used as pH optical sensors inside cell phagocytosed by murine macrophages.