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Imaging the cell wall of living single yeast cells using surface-enhanced Raman spectroscopy |
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| Authors: | Athiyanathil Sujith Tamitake Itoh Hiroko Abe Ken-ichi Yoshida Manikantan S Kiran Vasudevanpillai Biju Misturu Ishikawa |
| Institution: | (1) Present address: Department of Chemistry, National Institute of Technology Calicut, Calicut, 673601, Kerala, India;(2) Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu Kagawa, 761–0395, Japan |
| Abstract: | The surface of a living yeast cell (Saccharomyces cerevisiae strain W303-1A) has been labeled with silver (Ag) nanoparticles that can form nanoaggregates which have been shown to have surface-enhanced Raman scattering (SERS) activity. The cell wall of a single living yeast cell has been imaged by use of a Raman microspectroscope. The SERS spectra measured from different Ag nanoaggregates were found to be different. This can be explained on the basis of detailed spectral interpretation. The SERS spectral response originates from mannoproteins which cover the outermost regions of the yeast cell wall. Analysis of SERS spectra from the cell wall and the extracted mannoproteins from the yeast has been performed for the clarification of variation in SERS spectra. |
| Keywords: | Surface-enhanced Raman spectroscopy Living cell analysis Raman spectroscopic imaging Yeast cell wall Spectroscopy/Theory Laser spectroscopy Cell systems/Single cell analysis |
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