In vivo oximetry by a pulsed longitudinally detected ESR spectrometer |
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Authors: | H Yokoyama T Sato I Nicholson K Fukui H Ohya |
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Institution: | (1) Institute of Computer Science, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Krakow, Poland;(2) Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN 55455-0219, USA;(3) Division of Hematology, Oncology, and Transplantation, Department of Medicine, University of Minnesota Medical School, Minneapolis, MN 55455-0219, USA |
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Abstract: | By using a narrow single electron spin resonance (ESR) line agent, triarylmethyl, tris(8-carboxy-2,2,6,6-tetrahydroxyethylbenzo1,2-d:4,5-d′]
bis(1,3)dithiole-4-yl)methyl sodium salt (TAM OX063), pulsed longitudinally detected ESR (LODESR) measurements of a phantom
or the chest of a living mouse at the operating frequency of ca. 300 MHz were taken and the effective longitudinal relaxation
time (T
1*) was estimated for oximetry. Under irradiation of a pair of π-pulses with a variable interval between pulses (τ), in-phase
LODESR signal intensities were obtained from the phantoms containing TAM dissolved in a physiological saline solution at a
concentration of 1 mM and various concentrations of oxygen. TheT
1* of the phantom was calculated from the plotted curve of the LODESR signal intensity against τ. It was found that the reciprocal
ofT
1*, i.e., the longitudinal relaxation rate, increased with the concentration of oxygen. In vivo pulsed LODESR measurements of
the chest of living mice that had received a TAM injection via the intraperitoneal route were made. While the LODESR measurements
were being made, the mice in one group breathed normal air and those in another group breathed 100% oxygen. It was found that
the longitudinal relaxation rate of the mice breathing 100% oxygen was significantly greater than that of mice breathing normal
air, indicating that breathing 100% oxygen elevates the thoracic longitudinal relaxation rate. |
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