间二羧基苯氧基取代酞菁锌与白蛋白共价结合物的合成与光谱性质

制备了四种由β-四(3,5-二羧基苯氧基)酞菁锌(记为1)和β-八(3,5-二羧基苯氧基)酞菁锌(记为2)与人血清白蛋白(HSA)或牛血清白蛋白(BSA)构成的共价结合物,结合物中酞菁与白蛋白的摩尔组成比为6～7:1.在PBS溶液中的电子吸收光谱测试表明,当酞菁1共价结合到白蛋白上后,呈现出更明显的单体特征吸收(单体特...

Synthesis and spectroscopic properties of covalent albumin conjugates of phthalocyanine zincs substituted with metadicarboxyphenoxyl groups

Four kinds of covalent serum albumin (BSA or HSA) conjugates of beta-tetrakis[(3,5-dicarboxy)phenoxy]-phthalocyaninatozinc (1) and beta-octakis [(3, 5-dicarboxy) phenoxy]-phthalocyaninatozinc (2) were synthesized by the approach of amide bond. The molar ratio of phthalocyanine to albumin in conjugates were found to be 6 - 7.1. Their absorption spectra were measured in PBS solution. When conjugated to albumin, compound 1 displays more distinct monomer absorption characteristics (with the maximum absorption at 677 nm) than its free form. Compound 2 in albumin framework exists mostly in monomer form, which is beneficial to photodynamic therapy in aqueous solution. Both 2-BSA and 2-HSA show a sharp and intense Q-band at 681 nm and 682 nm with the molar extinction coefficient of 2.01 x 10(5) and 2.05 x 10(5) mol(-1) L cm(-1) respectively. The Q-band absorption spectra and existent state of phthalocyanine 1 or 2 in conjugates were not affected by the pH value of aqueous solution, which are obviously different from that of the corresponding free phthalocyanine.