| 适配体修饰金硒纳米合金共振散射光谱法检测痕量Hg2+ |
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| 引用本文： | Jiang ZL,Zhang Y,Qin HM,Zhou LP,Liang AH,Wang PF,Ouyang HX. 适配体修饰金硒纳米合金共振散射光谱法检测痕量Hg2+[J]. 光谱学与光谱分析, 2011, 31(5): 1371-1374. DOI: 10.3964/j.issn.1000-0593(2011)05-1371-04 |
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| 作者姓名： | Jiang ZL Zhang Y Qin HM Zhou LP Liang AH Wang PF Ouyang HX |
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| 作者单位： | 广西师范大学环境与资源学院,珍稀濒危动植物生态与环境保护省部共建教育部重点实验室,广西桂林541000 |
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| 基金项目： | 国家自然科学基金项目,广西自然科学基金项目,广西水利厅项目资助 |
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| 摘 要： | 用硼氢化钠还原法制备了金硒(AuSe)纳米合金.用单链核酸适配体(aptamer)修饰AuSe纳米合金制备了汞离子的核酸适配体纳米探针(Apta-AuSe).在pH 6.8 Na2HPO4-NaH2PO4缓冲溶液中及NaCl存在下,Apta-AuSe纳米探针亦不聚集;当Hg2+存在时,它可以稳定aptamer序列中的T...
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| 关 键 词： | 汞离子 核酸适配体 金硒纳米合金 共振散射光谱法 |
Resonance scattering detection of trace Hg2+ using aptamer modified AuSe nanoalloy |
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| Jiang Zhi-liang,Zhang Yi,Qin Hui-min,Zhou Lian-ping,Liang Ai-hui,Wang Peng-fei,Ouyang Hui-xiang. Resonance scattering detection of trace Hg2+ using aptamer modified AuSe nanoalloy[J]. Spectroscopy and Spectral Analysis, 2011, 31(5): 1371-1374. DOI: 10.3964/j.issn.1000-0593(2011)05-1371-04 |
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| Authors: | Jiang Zhi-liang Zhang Yi Qin Hui-min Zhou Lian-ping Liang Ai-hui Wang Peng-fei Ouyang Hui-xiang |
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| Affiliation: | Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, School of Environment and Resource, Guangxi Normal University, Guilin 541000, China. zljiang@mailbox.gxnu.edu.cn |
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| Abstract: | Under the condition of sodium citrate as stabilizer, the gold-selenium (AuSe) nano-alloy was prepared by sodium borohydride reduction procedure, and was modified by single-strand aptamer to obtain an aptamer nano-alloy probe (apta-AuSe) for Hg(II). In pH 6.8 Na2HPO4-NaH2PO4 buffer solution and in the presence of NaCl of 33 mmol L(-1), the Apta-AuSe probe is not aggregation. The apta-AuSe interacts with Hg2+ to form stable double-strand T-Hg(II)-T mismatches and to release AuSe nano-alloy particles from the probe. The released AuSe nano-alloy particles (20:1) aggregated to form bigger clusters that resulted in the resonance scattering (RS) intensity (I590 nm) increasing at 590 nm. The increased intensity delta I590 nm was proportional to the Hg2+ concentration from 1.3 to 1466 nmol L(-1), with a detection limit of 0.74 nmol L(-1). The regress equation was delta I590 nm = 0.603c + 2.0. Thus, a new resonance scattering (RS) spectroscopy of apta-AuSe was applied to the analysis of trace mercury ion. This simple, rapid, selective and sensitive aptamer AuSe nano-alloy RS assay was applied to the determination of Hg2+ in wastewater, with satisfactory results. |
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