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| 单分子层修饰生物芯片基片的电化学研究 | |
| 引用本文： | 梁振普,宋安东,谢斌. 单分子层修饰生物芯片基片的电化学研究[J]. 武汉大学学报(理学版), 2005, 51(2): 249-252 |
| 作者姓名： | 梁振普 宋安东 谢斌 |
| 作者单位： | 1. 河南农业大学 生命科学学院, 河南 郑州,450002 2. BT Biomedical Technology AB,Lund 22370,Sweden 3. Department of Pure and Applied Biochemistry,Lund University, Lund 22100,Sweden |
| 基金项目： | 国家863计划资助项目(2001AA514020 02) |
| 摘 要： | 构建了一种亲和素单分子层修饰的电化学分析生物芯片基片，并且以二茂铁和亚铁氰化钾为指示剂对该基片的电化学性能进行了研究．循环伏安实验结果表明，单分子层的存在增强了二茂铁在基片上的响应电流，二茂铁可以作为该基片的高效电子媒介体，实验结果具有良好的重现性和一致性，基片产生的背景噪声可以忽略不计．文中对这些结果产生的原因进行了讨论． |
| 关 键 词： | 生物芯片 单分子层 生物素-亲和素系统 电化学 |
| 文章编号： | 1671-8836(2005)02-0249-04 |
| 修稿时间： | 2004-10-20 |
Electrochemistr Study on Monolayers Modified Biochips |
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| LIANG Zhen-pu,SONG An-dong,Michael Mecklenburg,Bengt Danielsson,XIE Bin. Electrochemistr Study on Monolayers Modified Biochips[J]. JOurnal of Wuhan University:Natural Science Edition, 2005, 51(2): 249-252 | |
| Authors: | LIANG Zhen-pu SONG An-dong Michael Mecklenburg Bengt Danielsson XIE Bin |
| Affiliation: | LIANG Zhen-pu~1,SONG An-dong~1,Michael Mecklenburg~2,Bengt Danielsson~3,XIE Bin~3 |
| Abstract: | A biotin-avidin modified biochip has been constructed, and its electrochemistry characteristics were studied with K_4Fe(CN)_(6).3H_2O and ferrocene as the indicators by cyclic voltammograms. Electrochemical experiments showed that this biochip has high response signal, reproducibility,uniformity and low background signal; the ferrocene can be used as high efficiency electron medium. And these results were discussed in this paper. |
| Keywords: | biochips monolayers biotin-avidin system electrochemistry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |