| 纳米金对荧光素的荧光增效作用 |
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| 引用本文： | 余林海,莫志宏,万巧玲,钱俊臻.纳米金对荧光素的荧光增效作用[J].分析化学,2006,34(9):1307-1310. |
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| 作者姓名： | 余林海 莫志宏 万巧玲 钱俊臻 |
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| 作者单位： | 1. 重庆大学化学化工学院,重庆,400044
2. 重庆大学化学化工学院,重庆,400044;重庆大学微系统研究中心,重庆,400044 |
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| 摘 要： | 纳米金对荧光素的荧光效率具有增强作用,其增强效果取决于纳米金的尺寸大小和浓度。粒径分别为5、15、25 nm的纳米金与不同浓度的荧光素溶液作用后可以增强荧光强度3~10倍,同时讨论了溶液环境和pH值对荧光增强的影响。采用本实验提出的方法可以在生化检测方面提高荧光检测方法的灵敏度。
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| 关 键 词： | 纳米金 荧光增强 生化检测 |
| 收稿时间： | 10 31 2005 12:00AM |
| 修稿时间： | 2005-10-312005-12-19 |
Development of Fluorenscence Detection with Nanogold-Enhancement |
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| Yu Linhai,Mo Zhihong,Wan Qiaoling,Qian Junzhen.Development of Fluorenscence Detection with Nanogold-Enhancement[J].Chinese Journal of Analytical Chemistry,2006,34(9):1307-1310. |
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| Authors: | Yu Linhai Mo Zhihong Wan Qiaoling Qian Junzhen |
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| Abstract: | Abstracd Nanogold with different sizes was utilized to enhance the quantum yields in fluorescein solution,which could improve the sensitivity of the biomolecular detection greatly.This enhancement effect was dependent on the size and the concentration of the gold nanoparticle,in particular,higher enhancement results were obtained with 15 nm gold nanoparticle compared to 5 nm and 25 nm gold nanoparticles.The effect of pH and ion stength to fluorescence was investigated in this article,The phenomena were explained with quantum theory and adsorption theory.The lower deoxyribonucleic acid(DNA) detection limit was obtained with this method. |
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| Keywords: | Gold nanoparticle fluorescence enhancement biochemical detect |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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