Determination of sialic acids released from glycoproteins using capillary zone electrophoresis/electrospray ionization mass spectrometry |
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Authors: | Ortner Karin Buchberger Wolfgang |
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Institution: | Institute of Analytical Chemistry, Johannes-Kepler-University, Linz, Austria. |
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Abstract: | A simple method for the determination of the two most abundant sialic acids released from glycoproteins based on CZE-MS is presented. Several parameters like BGE with various organic modifiers and sheath liquids were studied with respect to their suitability for the fast and easy analysis of the selected compounds by CZE-MS. Finally, a BGE containing 10 mM ammonium acetate allowed the quantification of N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) in glycoproteins as well as human plasma samples. LOD and LOQ were 2 microg/mL and 6 microg/mL, respectively. |
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