SPECTRAL CHARACTERISTICS AND MECHANISM OF CHEMILUMINESCENCE FROM TRYPTOPHAN SOLUTIONS INDUCED BY UV-IRRADIATION |
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Authors: | Janusz Slawiski Marian Elbanowski‡ Danuta Slawiska |
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Institution: | Institute of Physics and Chemistry, Academy of Agriculture, Wojska Polskiego 38/42, 60–637 Pozná.;?Institute of Chemistry, Pozná, University, Grunwaldzka 6, 60–780 Pozna, Poland |
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Abstract: | The spectral distribution of the chemiluminescence, fluorescence and phosphorescence of tryptophan aqueous solutions irradiated with high and low pressure mercury lamps has been measured. The blue emission bands in the region of 380–520 nm observed both in the chemi- and photoluminescence, as well as an absorbance increase at 230 and 330 nm, indicate oxidative degradation of tryptophan leading to the formation of derivatives of N-formylkynurenine, xanthurenic and anthranilic acids. Red emission bands at 630 and 705 nm in the spectrum of the chemiluminescence, an enhancement of light intensity by D2O and its decrease by NaN3 and DABCO suggest a partial contribution of O2(1Δg) to the photooxidation and chemiluminescence of tryptophan. The enthalpy of the exergonic reactions, leading to the formation of luminescing products, was calculated to average -270 kJ-mol. |
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