| 鼠白细胞介素2真核表达质粒的构建及鉴定 |
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| 引用本文： | 张富春,马正海,等.鼠白细胞介素2真核表达质粒的构建及鉴定[J].新疆大学学报(理工版),2001,18(3):280-283. |
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| 作者姓名： | 张富春 马正海 |
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| 作者单位： | [1]新疆大学生命科学与技术学院分子生物学重点开放实验室,新疆乌鲁木齐830046 [2]新疆大学生命科学与技术学院分子 |
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| 基金项目： | 国家"863"高技术青年基金和国家自然科学基金(39960047)资助 |
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| 摘 要： | 利用小鼠IL－2基因构建真核表达质粒,筛选的阳性克隆经限制性内切酶酶切鉴定和基因测序,结果表明小鼠IL－2基因被正确地克隆支pCDNA3真核表达质粒上,将真核心质粒pCDNA2IL-2,转化大肠杆菌细胞,增菌培养后大量提取pCDNA3IL-2质粒,经紫外分光光度计检测其纯度和浓度,为加强控制害DNA疫苗的免疫功能奠定了科学的理论基础。
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| 关 键 词： | pCDNA3IL－2 白细胞介素2 真核心表达质粒 基因测序 T细胞生长因子 免疫功能 |
| 文章编号： | 1000-2839(2001)03-0280-04 |
| 修稿时间： | 2000年12月20 |
Constraction and Identification of Eukarytic Express Plasmid of Murine IL-2 |
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| ZHANG Fu-chun,MA Zheng-hai,MA Ji,GUO Jiang-yong,ZHONG Zhe.Constraction and Identification of Eukarytic Express Plasmid of Murine IL-2[J].Journal of Xinjiang University(Science & Engineering),2001,18(3):280-283. |
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| Authors: | ZHANG Fu-chun MA Zheng-hai MA Ji GUO Jiang-yong ZHONG Zhe |
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| Abstract: | The murine interleukin-2(IL-2) is an important immunoregulatory factor that can be used as the immunological adjuvant in mouse model.The murine IL-2 gene has been cloned into eukaryotic express plasmid pCDNA\-3 after identification of recombinant and sequencing.The positive colne is named pCDNA\-3LI-2.This plasmid was turned into E.coll.which was cultured overnight.Then the plasmid was substracted and purificated and the purification and concentration were examined with spectraphctometer. |
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| Keywords: | murine IL-2 sequencing plasmid pCDNA\-3IL-2 |
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