| CULTURE AND REGENERATION OF POPLAR MESOPHYLL PROTOPLASTS |
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| 引用本文： | 王善平,许智宏,卫志明. CULTURE AND REGENERATION OF POPLAR MESOPHYLL PROTOPLASTS[J]. 中国科学B辑(英文版), 1991, 0(5) |
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| 作者姓名： | 王善平 许智宏 卫志明 |
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| 作者单位： | Shanghai Center of Biotechnology，Academia Sinica.，Plant Molecular Genetics Lab，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai 200031，PRC，Plant Molecular Genetics Lab，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai 200031，PRC |
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| 基金项目： | This research is granted by the National Biotechnology Programme. |
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| 摘 要： | Populus tomentosa is one special species of poplar growing in North China. Mesophyllprotoplasts were isolated from the axenic shoots and cultured in the modified KM8p liquidmedium. Protoplast-derived cells started to divide after 7 days of culture. The frequencyof cell division reached about 20% in 10 days. The yellowish green calli grew compact andnodular after the hormone concentration of medium was adjusted. Shoot formation occurredwhen the protoplast--derived calli were transferred onto MS medium containing zeatin andIAA or NAA. The shoots rooted readily on 1/2 MS hormone--free medium.
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CULTURE AND REGENERATION OF POPLAR MESOPHYLL PROTOPLASTS |
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| WANG SHAN-PING,XU ZHI-HONG,WEI ZHI-MING Plant Molecular Genetics Lab. CULTURE AND REGENERATION OF POPLAR MESOPHYLL PROTOPLASTS[J]. Science in China(Chemistry), 1991, 0(5) |
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| Authors: | WANG SHAN-PING XU ZHI-HONG WEI ZHI-MING Plant Molecular Genetics Lab |
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| Affiliation: | WANG SHAN-PING,XU ZHI-HONG,WEI ZHI-MING Plant Molecular Genetics Lab,Shanghai Institute of Plant Physiology,Academia Sinica,Shanghai 200031,PRC Shanghai Center of Biotechnology,Academia Sinica. |
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| Abstract: | Populus tomentosa is one special species of poplar growing in North China. Mesophyllprotoplasts were isolated from the axenic shoots and cultured in the modified KM8p liquidmedium. Protoplast-derived cells started to divide after 7 days of culture. The frequencyof cell division reached about 20% in 10 days. The yellowish green calli grew compact andnodular after the hormone concentration of medium was adjusted. Shoot formation occurredwhen the protoplast--derived calli were transferred onto MS medium containing zeatin andIAA or NAA. The shoots rooted readily on 1/2 MS hormone--free medium. |
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| Keywords: | Populus tomentosa protoplast culture plant regeneration |
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