Transverse pore gradient gel electrophoresis of lipoprotein [a] using methylenebisacrylamide gradients. |
| |
Authors: | G B Smejkal H F Hoff |
| |
Affiliation: | Cleveland Clinic Foundation Research Institute, Department of Vascular Cell Biology and Atherosclerosis Research, OH 44195. |
| |
Abstract: | We describe a modification of transverse pore gradient gel electrophoresis in which pore size is regulated by crosslinker proportion (%C) rather than by total monomer concentration (%T). We electrophoresed plasma lipoprotein [a] transversely across linear N,N'-methylenebisacrylamide gradients and measured mobility as a function of %C. This method allows for the simultaneous assessment of pore sizes generated over a wide range of crosslinker proportions. |
| |
Keywords: | |
|
|