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Determination of glutamate pyruvate transaminase and pyruvate with an amperometric pyruvate oxidase sensor |
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| Authors: | Fumio Mizutani Keishiro Tsuda Isao Karube Shuichi Suzuki Kunio Matsumoto |
| Institution: | Research Institute for Polymers and Textiles, 1-1-4, Yatabe-Higashi, Tsukuba, Ibaraki-ken 305 Japan;Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Nagatsutacho, Midori-ku, Yokohama 227 Japan;Toyo Joyo Co., Mifuku, Ohito-cho, Shizuoka-ken 410-23 Japan |
| Abstract: | Pyruvate oxidase (E.C. 1.2.3.3.) is immobilized by adsorption on a porous acetylcellulose membrane, and combined with an oxygen electrode to provide a sensor for pyruvate (0.1–0.8 mM). The response time is 2 min. Glutamate pyruvate transaminase (0.5–180 × 10-3 I.U. ml-1) is determined by its effect on pyruvate production by the alanine—α-ketoglutarate reaction. The sensor is stable for more than 10 days and 100 assays. |
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