Cadmium speciation in Arabidopsis thaliana as a strategy to study metal accumulation system in plants |
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Authors: | Kasia Po?e?-Pawlak Rafa? Ruzik Monika Ciurzyńska |
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Institution: | a Department of Analytical Chemistry, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland b Department of Pomology and Basic Natural Sciences in Horticulture, Warsaw Agricultural University, Nowoursynowska 166, 02-787 Warsaw, Poland |
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Abstract: | The strategy to identify cadmium deactivation mechanism in Arabidopsis thaliana has been developed using selective and sensitive hyphenated techniques. Cadmium concentrations, in main parts of the plant, were determined by ICP-MS and total amount was found as 0.43-0.44 μg g−1 in leaves and 3.3-3.4 μg g−1 in roots. Speciation of the metal complexes in cells was investigated by SEC-ICP-MS in order to estimate the accumulation process. Phytochelatins, desglycyl-phytochelatins and phytochelatins homologues lacking the N-terminal γ-linked glutamic acid were extracted from plant and were identified by RPLC-ESI-MS. Two-dimensional chromatography allowed to link the metal complexes separated by SEC with isoforms of phytochelatins analyzed by high resolution RPLC and confirm their significant responsibility for metal accumulation. The potential of the cadmium complexes speciation indicates that obtained results could be reliable source of knowledge to confirm the information coming from the well-known genomic sequence of Arabidopsis and to estimate the role of γ-glutamyl transpeptidase in metabolism of glutathione. |
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Keywords: | Cadmium accumulation Phytochelatins Reversed phase liquid chromatography Size exclusion chromatography ICP-MS Electrospray MS Hyphenated techniques |
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