Comparison of column properties in reversed-phase chromatography: monolithic, cholesterolic and mixed bonded stationary phases |
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Authors: | Katarzyna Krupczyńska Bogus?aw Buszewski |
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Affiliation: | a Department of Environmental Chemistry and Ecoanalytics, Faculty of Chemistry, Nicholas Copernicus University, 7 Gagarin St., 87100 Toruń, Poland b Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Nám. ?s. Legií 565, CZ-53210 Pardubice, Czech Republic |
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Abstract: | A commercial Chromolith C18 column and two new stationary phases with mixed ligands bonded on the Kromasil silica gel support, SG-MIX and SG-Chol, were characterized using simple tests based on the retention of non-polar, basic and acidic compounds. Polar and methylene selectivity tests in acetonitrile-water and methanol-water mobile phases revealed lower hydrophobicities of the SG-MIX and SG-Chol columns in comparison to the Chromolith column. The columns were further characterized using new test criteria - gradient oligomer capacity and isomeric selectivity and peak symmetry of naphthalene di-sulphonic acids in aqueous mobile phases. The cholesterolic column shows greater gradient oligomer selectivity for the separation of oligoethylene glycol samples than the SG-MIX and the Chromolith columns. Increased retention and peak tailing, but decreased isomeric selectivity for naphthalene-di-sulphonic acids was observed with the SG-MIX column, because of interactions with various polar bonded groups. |
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Keywords: | Column characterization Bonded phase Selectivity Naphthalene sulphonic acids Oligoethylene glycols |
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