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Mucin/carbopol matrix to immobilize oxalate oxidase in a urine oxalate amperometric biosensor |
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| Authors: | RH Capra PM Vadgama |
| Institution: | a INFIQC, Departamento de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina b IMBIV, Departamento de Química Orgánica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina c IRC in Biomedical Materials, Queen Mary, University of London, England d Hospital Privado Centro Médico de Córdoba, Argentina |
| Abstract: | An enzymatic amperometric electrode with extended analytical range and improved stability for oxalate determination has been developed. Glutarlaldehyde/mucin/carbopol matrix was used for the crosslinking of the enzyme between polymeric membranes to form a classical laminate construction (sandwich) and compared with the glutaraldehyde/mucin/enzyme and glutaraldehyde/albumin/enzyme.The use of a sulphonated membrane as internal membrane allowed rejection of the most important electrooxidable urine interferents. The recovery assays were highly satisfactory. The wide linear response in the range 2-400 μM after 1/10 urine dilution (corresponding to 20-4000 μM) made it suitable for clinical range. High correlation with the standard spectrophotometric method was obtained (r2 = 0.98, y = 0.89x, n = 25). |
| Keywords: | Mucin Carbopol Oxalate biosensor Amperometric biosensor Oxalate oxidase |
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