Development of a class selective immunoassay for the type II pyrethroid insecticides |
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Authors: | Sally K. Mak Hu-Jang Lee Bruce D. Hammock |
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Affiliation: | Department of Entomology and Cancer Research Center, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA |
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Abstract: | A general and broad class selective enzyme-linked immunosorbent assay was developed for the type II pyrethroid insecticides, such as cypermethrin, deltamethrin, cyhalothrin, cyfluthrin, fenvalerate, esfenvalerate and fluvalinate. Polyclonal antibodies were generated by immunizing with a type II pyrethroid immunogen ((RS)-α-cyano-3-phenoxybenzyl (RS)-cis,trans-2,2-dimethyl-3-carboxyl-cyclopropanecarboxylate) conjugated with thyroglobulin. Antisera were screened against nine different coating antigens. The antibody-antigen combination with the most selectivity for type II pyrethroids such as cypermethrin was further optimized and tested for tolerance to co-solvent, pH and ionic strength changes. The IC50s of the optimized immunoassay were 78 μg l−1 for cypermethrin, 205 μg l−1 for cyfluthrin, 120 μg l−1 for cyhalothrin, 13 μg l−1 for deltamethrin, 6 μg l−1 for esfenvalerate, 8 μg l−1 for fenvalerate and 123 μg l−1 for fluvalinate. No cross-reactivity was measured for the type I pyrethroids such as permethrin, bifenthrin, phenothrin, resmethrin and bioresmethrin. This assay can be used in monitoring studies to distinguish between type I and II pyrethroids. |
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Keywords: | Pyrethroid Insecticide Pesticide class Type II Immunoassay ELISA |
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