|
|||||
|
|
Detection of d-serine in neural samples by saccharide enhanced chiral capillary electrophoresis |
|
| Authors: | Zhe Quan Yaru Song Michael H. LeBlanc |
| Affiliation: | a Department of Chemistry, Jackson State University, 1400 Lynch Street, Jackson, MS 39217, USA b Department of Pharmacology and Toxicology, The University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216, USA c Department of Pediatrics, The University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216, USA |
| Abstract: | Highly fluorescent enantiomeric derivatives of d/l-serine from naphthalene-2,3-dicarboxaldehyde were resolved by hydroxypropyl-γ-cyclodextrin modified capillary electrophoresis using a saccharide as an enhancing chiral selector. Four saccharides, i.e. d-(+)-glucose, d-(−)-fructose, β-lactose, and sucrose were tested. Similar enhancing effects were observed. Coupled with laser induced fluorescence detection, this separation was applied to the determination of d-serine in neural samples including rat brain tissues, Aplysia ganglia, microdialysates from rat brain, and Aplysia individual neurons. High levels of d-serine were found in certain rat brain sections including hippocampus and striatum. d-Serine was also found to occur in Aplysia ganglia, but interestingly, it was not detected in single neurons isolated from Aplysia ganglia. |
| Keywords: | smallcaps" >d-Serine Capillary electrophoresis Enantiomeric separation Rat brain tissue Microdialysates Aplysia ganglia |
| 本文献已被 ScienceDirect 等数据库收录！ | |