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Mass spectrometry imaging of small molecules from live cells and tissues using nanomaterials |
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| Authors: | Jae Young Kim Heejin Lim Dae Won Moon |
| Institution: | 1. School of Electronic and Electrical Engineering, College of IT Engineering, Kyungpook National University Daegu Campus, Daegu, Republic of Korea;2. Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, Republic of Korea |
| Abstract: | We review recently developed methods for analyzing live cells and tissues in ambient conditions without the use of harsh chemical fixation or physical freezing and drying. The first method is based on laser ablation in atmospheric pressure assisted by atmospheric pressure plasma and nanomaterials such as nanoparticles and graphene to enhance laser ablation. The second method is based on secondary ion mass spectrometry imaging of live cells in solution capped with single-layer graphene to preserve intact and hydrated biological samples even under ultrahigh vacuum for secondary ion mass spectrometry bio-imaging in solution with subcellular spatial resolution. Mass spectrometry imaging of small molecules from live cells and tissues can provide an innovative molecular imaging methodology for several biomedical and material research applications. |
| Keywords: | ambient mass spectrometry imaging live cells and tissues nanomaterials secondary ion mass spectrometry |