Starter units of the biosynthesis of blepharismins: self-defense pigments of Blepharisma japonicum |
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Authors: | Koichi Yoshioka Yoshiyuki Uruma Hideo Iio |
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Affiliation: | a Department of Material Science, Graduate School of Science, Osaka City University, Osaka 558-8585, Japan b Department of Materials Science, Yonago National College of Technology, Tottori 683-8502, Japan |
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Abstract: | The heterotrich ciliate Blepharisma japonicum produces red pigment blepharismins, which function as self-defense toxin against predators and as a photoreceptor for step-up photonegativity. The dibenzoperylenequinone moiety of blepharismins was shown to be biosynthesized via the polyketide pathway. In this paper, the starter units of the biosynthetic pathway of blepharismins were determined to be isovaleryl-CoA and butyryl-CoA by HPLC, LC/ESI-MS, and 1H and 2H NMR analyses of the pigments obtained from feeding experiment of l-leucine or sodium butyrate in excess and deuterium-labeled l-leucine. |
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Keywords: | Blepharismin Biosynthesis Starter unit Isovaleryl-CoA smallcaps" >l-Leucine-d10 |
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