碳纤维表面特性对反硝化菌固着化的影响

通过对比反硝化菌在不同基体碳纤维表面的固着化及碳纤维表面含氧官能团对菌种的固着化实验,研究了碳纤维(基体、比表面积、循环利用性等)特性,尤其表面含氧官能团对反硝化菌固着化的影响。实验结果表明,反硝化菌在PAN基碳纤维上的平均挂膜厚度明显高于沥青基和粘胶基碳纤维。碳纤维表面适量的含氧官能团有助于较多、较牢固地固着反硝化菌。对三种PAN基碳纤维的XPS谱图分析表明碳纤维表面含氧官能团—O—CO对反硝化菌的固着化影响较大,当碳纤维表面含氧官能团—O—CO中碳原子占表面碳原子总量的3%左右时反硝化菌固着化效果较佳。经过比较表明PAN基碳纤维是一种生物相容性好、固着化程度高、再生能力强、耐微生物分解及化学腐蚀的优异的新型反硝化菌固着化载体。

Effect of surface characteristics of carbon fiber on immobilization of denitrifying bacterium

By means of contrasted immobilization of denitrifying bacterium on several kinds of carbon fibers, the effect of physical and chemical characteristics such as specific surface area, precursor and recycle, especially the surface oxygenic functional group on these carbon fibers was investigated. Study showed that the immobilization of microorganism on polyacrylonitrile (PAN) base carbon fiber was better than those on pitch base and viscose base carbon fiber. And while the surface oxygenic functional groups of carbon fiber must be kept proper content, microorganism could be fixed more and firmly on the surface of carbon fiber. X ray photoelectron spectrum (XPS) analysis about the three kinds of PAN base carbon fibers revealed that the influence of the oxygenic functional group O-C O was the most important for the immobilized biomass; and the immobilized biomass was the most while the content of -O-C O was about 3%. PAN base carbon fiber with high strength was a kind of excellent carrier with highly immobilized ability and resisting chemical erosion, decomposition of microorganism and strong hydraulic impaction for denitrifying biofilm.