Chromatographic behaviour and purification of N1.5-glycosyl enkephalins |
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Authors: | J. L. Torres P. Clapés I. Haro G. Valencia F. Reig J. M. García Antón |
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Affiliation: | (1) Peptide Research Section, Dep. of Biological Organic Chemistry, C.I.D., C.S.I.C., c/Jordi Girona, 18-26, E-08034 Barcelona, Spain |
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Abstract: | Summary N1.5-Glycosyl enkephalins are potent analgesics. Two enkephalin-related glycosyl peptides: [DMet2, Pro5] enkephalin (N1.5 -glucopyranosyl] amide and [DMet2, Pro5] enkephalin [N1.5 -galactosyl] amide have been prepared following the liquid phase procedure. The chromatographic behavior of both the final glycosyl enkephalins and their synthetic intermediates on reversed-phase columns has been tested. Semipreparative, reversed-phase, HPLC conditions have been developed in order to obtain a simple and rapid procedure for the purification of the two synthetic pentapeptides. Thus, using isocratic elution, injections of 10mg of crude material after gel filtration gave homogeneous (99.9%) glycosyl enkephalins. |
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Keywords: | Column liquid chromatography Analgesic peptides Opioids Glycopeptides Glycosylenkephalins |
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