| 壳聚糖-(4-叔丁基邻苯二酚)的酪氨酸酶催化接枝初探 |
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| 引用本文： | 薛丽群,罗志敏,王晓琳,陈盛. 壳聚糖-(4-叔丁基邻苯二酚)的酪氨酸酶催化接枝初探[J]. 广州化学, 2009, 34(1): 32-35 |
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| 作者姓名： | 薛丽群 罗志敏 王晓琳 陈盛 |
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| 作者单位： | 福建师范大学,福清分校,福建,福清,350300;福建师范大学,福清分校,福建,福清,350300;福建师范大学,福清分校,福建,福清,350300;福建师范大学,福清分校,福建,福清,350300 |
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| 摘 要： | 以自制马铃薯酪氨酸酶催化氧化4-叔丁基邻苯二酚成活性邻醌，后与壳聚糖接枝反应，并着重探讨了反应条件对接枝率的影响。通过红外光谱对该反应产物进行结构的初步表征，结果表明酶催化接枝反应的适宜条件为：4-叔丁基邻苯二酚浓度3．0mmol／L、酶活力20U／mL、反应时间30min、壳聚糖溶液百分含量0．5％；用线性电位滴定法测定其接枝率为37％～64％。
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| 关 键 词： | 壳聚糖 酪氨酸酶 4-叔丁基邻苯二酚 |
Chitosan-(4-tert-butylcatechol) Catalyzed and Grafted by Tyrosinase |
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| XUE Li-qun,LUO Zhi-min,WANG Xiao-lin,CHEN Sheng. Chitosan-(4-tert-butylcatechol) Catalyzed and Grafted by Tyrosinase[J]. Guangzhou Chemistry, 2009, 34(1): 32-35 |
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| Authors: | XUE Li-qun LUO Zhi-min WANG Xiao-lin CHEN Sheng |
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| Affiliation: | Fuqing Branch of Fujian Normal University;Fuqing Fujian 350300;China |
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| Abstract: | 4-tert-butylcatechol was catalyzed and oxidized by potato tyrosinase to obtain reactive o-quinones,which was grafted onto chitosan.The structure of the reactant was characterized by FTIR.The effect of reaction conditions on grafting rate was investigated.The optimal conditions for grafting reaction were determined:4-tert-butylcatechol concentration 3.0 mmol/L,tyrosinase activity 20 U/mL,reaction time 30 min and chitosan solution percentage 0.5%.The grafting rate was from 37 % to 64 % determined by linear ti... |
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| Keywords: | chitosan tyrosinase 4-tert-butylcatechol |
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