气相色谱-质谱分析新种海绵Reniochalina sp.中的低极性组分

采用气相色谱-质谱联用技术（GC-MS）与核磁共振氢谱（1H-NMR）相结合的方法,对中国黄海肾指海绵属新种海绵Reniochalina sp.中的低极性组分进行了研究。实验首先采用环己烷对海绵进行提取，然后将提取液浓缩，用硅胶柱对其进行柱色谱分离。取乙酸乙酯-环己烷（体积比为1∶9）混合液梯度洗脱所得油状物用于连续两次制备色谱分离，得到两个样品，对其进行1H-NMR及GC-MS分析。此次实验共从该海绵中分离鉴定出10种链烃、2种脂肪酸、2种脂肪酸酯、邻苯二甲酸以及3种邻苯二甲酸二酯共18种化学成分。该研

Analysis of Low-Polarity Constituents of Reniochalina sp.by Gas Chromatography-Mass Spectrometry

The sponge of Reniochalina sp. was collected from China Yellow Sea. The specimen (dry weight 100 g) was repeatedly extracted with cyclohexane. The combined crude extract was then evaporated in vacuo, and the dark red residue (5 g) was subjected to a silica gel column for chromatographic separation using gradient mixtures of ethyl acetate and cyclohexane as eluents. The fraction eluted with ethyl acetate-cyclohexane (1:9, v/v) was repeatedly separated by preparative liquid chromatography (HiQ sil C18-10 column, 250 mm x 21.2 mm i.d. ) to afford sample B and sample C as dark red oil. These two low-polarity samples were analyzed by gas chromatography-mass spectrometry (GC-MS) equipped with an HP-5 column (30 m x 0.25 mm i.d.). The oven temperature was raised from 40 degrees C to 250 degrees C at 10 degrees C /min and kept at 250 degrees C for 70 min. Using GC-MS coupled with 1H-nuclear magnetic resonance (1H-NMR), 18 compounds including alkanes, alkenes, fatty acids, fatty acid esters, phthalic acid and phthalic esters, were identified. The results provide to be helpful for in taxonomy of Reniochalina.