Epicatechin-copper 9II) complexes: Damage of small intestinal epithelium |
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Authors: | Ruxandra B Stavrescu Takahide Kimura Fumiko Hayakawa Takashi Ando |
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Institution: | (1) Department of Chemistry, Shiga University of Medical Science, Seta, Otsu, 520-2192 Shiga, Japan;(2) Department of Life Style Studies, School of Human Cultures, The University of Shiga Prefecture, Hikone, 522-8533 Shiga, Japan |
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Abstract: | Four epicatechins (−)-epicatechin (EC), (−)-epicatechin gallate (ECg), (−)-epigallocatechin (EGC), (−)-epigallocatechin gallate
(EGCg)] and their corresponding copper complexes were compared with regard to their effect on the viability of Caco-2 colon
cancer cells in vitro, measured by 3-(4,5-dimethylthyazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay. The viability
of Caco-2 cells exposed to EC (1 mM), ECg (1 mM) or EGC (1mM) respectively, for 30 min, was comparable to that of the saline
control group, while EGCg (1 mM) apparently enhanced cellular activity. in contrast, the cells treated with epicatechin-copper
complexes were killed. Bivalent copper 91 mM), in similar conditions, did not affect the cells. No cell leakage or other histological
differences were observed, implying a rapid cell death. The suggested mechanism of killing is by OH radical attack, produced
in the presence of epicatechin-copper complexes, but not in the presence of either of the epicatechins or copper alone. The
reaction sites are discussed. |
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Keywords: | Caco-2 copper epicatechin intestinal absorption MTT assay oxidative stress Wilson's disease |
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