Simultaneous spectrophotometric and liquid chromatographic determination of dextromethorphan hydrobromide,phenylephrine hydrochloride,and carbinoxamine maleate in pharmaceutical preparations |
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Authors: | Ismail Murat Palabiyik Feyyaz Onur |
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Institution: | 1.Department of Analytical Chemistry, Faculty of Pharmacy,University of Ankara Tandogan,Ankara,Turkey |
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Abstract: | Simultaneous determination of dextromethorphan hydrobromide (DEX), phenylephrine hydrochloride (PHEN), and carbinoxamine maleate (CAR) in pharmaceutical preparations was performed by using liquid chromatograpy (LC) and spectrophotometry. In LC, the separation
was achieved on a C18 column and the optimal mobile phase for satisfactory separation in a gradient elution program was found
to be acetonitrile-sodium perchlorate solution (5: 95, v/v) initially, then a linear gradient up to 60% acetonitrile in 8
min. In spectrophotometric method, a chemometric technique, principal component regression (PCR), was used. In the method,
the absorbance data matrix corresponding to the concentration data matrix was obtained by the measurement of absorbances in
their zero order spectra by Δλ = 1 nm in the 210–300 nm range. Then, the calibration was obtained by using this data matrix
for the prediction of unknown concentrations of DEX, PHEN, and CAR in their ternary mixture. The methods proposed were validated
and successfully applied to a pharmaceutical preparation, capsule, and the results were compared. |
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