| 16S rDNA-RFLP方法分析抑菌土中的细菌多样性 |
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| 引用本文： | 周峻沛,邹长松,顾英琦,莫明和.16S rDNA-RFLP方法分析抑菌土中的细菌多样性[J].云南大学学报(自然科学版),2007,29(4):424-429,432. |
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| 作者姓名： | 周峻沛 邹长松 顾英琦 莫明和 |
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| 作者单位： | 云南大学,省部共建生物资源保护与利用重点实验室培育基地,云南,昆明,650091 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，国家自然科学基金，云南省科技厅资助项目 |
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| 摘 要： | 采集了从云南省16个县烟草主栽区的土壤样品129份.通过测定土壤抑制线虫生防菌Pochonia chlamydosporia孢子萌发率的大小将土样聚为抑菌率极显著差异的5个类群(P >0.01).为研究抑菌土中的细菌种群,构建了强抑菌土样的细菌16S rRNA基因文库;利用限制性片段长度多态性(RFLP)对随机克隆进行筛选;测定代表克隆的16S rDNA序列;对强抑菌土中的细菌种群进行了系统发育分析,并表明Proteobacteria和Acidobacteria的细菌是强抑菌土中的主要细菌类群.
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| 关 键 词： | 土壤抑真菌作用 细菌群落 生物防治 16SrRNA |
| 文章编号： | 0258-7971（2007）04-0424-06 |
| 修稿时间： | 2007-02-27 |
Phylogenetic diversity of bacteria in fungistasis soil determined by 16S rDNA-RFLP analysis |
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| ZHOU Jue-pei, ZOU Chang-song, MO Ming-he.Phylogenetic diversity of bacteria in fungistasis soil determined by 16S rDNA-RFLP analysis[J].Journal of Yunnan University(Natural Sciences),2007,29(4):424-429,432. |
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| Authors: | ZHOU Jue-pei ZOU Chang-song MO Ming-he |
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| Institution: | Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming 650091, China |
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| Abstract: | For the objective to study bacterial community related to soil fungistasis,129 soil samples were collected from tobacco fields in 16 counties of Yunnan Province.After testing the fungistatic capability of inhibit conidialgermination of nematophagous fungus Pochonia chlamydosporia,these samples were combined into 5 significant groups(P>0.01) according to their fungistatic values.The 16S rRNA gene library of strong fungistatic soil was constructed and random clones were screened by RFLP method.Phylogenetic analysis on bacterial community showed that members of Proteobacteria and Acidobacteria were dominant bacteria in fungistatic soil. |
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| Keywords: | 16S rRNA |
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