Highly sensitive microRNA quantification with zero background signal from silver nanoparticles |
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| Institution: | 1. CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, PR China;2. University of Chinese Academy of Sciences, Beijing 100049, PR China;3. Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai, 200444, PR China;1. Analytical Chemistry, Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, Bochum D-44780, Germany;2. Center for Electrochemical Sciences (CES)–Molecular Nanostructures, Ruhr-Universität Bochum, Bochum D-44780, Germany;1. School of Life Science and Technology, China Pharmaceutical University, Nanjing, China;2. Simcere Pharmaceutical Co., Ltd., Nanjing, Jiangsu, China;3. BioSciKin Co., Ltd., Nanjing, Jiangsu, China;4. MtC BioPharma Co., Ltd., Nanjing, Jiangsu, China |
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| Abstract: | MicroRNAs are a class of noncoding RNAs, which play vital roles in numerous cellular processes. Recent studies have confirmed their significance in the theranostics of various diseases. We herein fabricate an electrochemical approach for microRNA quantification. DNA/microRNA/DNA hybridization and electrochemical signals from silver nanoparticles (AgNPs) are employed in this work. DNA1 immobilized on a gold electrode interacts with target microRNA, along with amino group labeled DNA2, to form the sandwich hybrid. The adjacent DNA1 and DNA2 are then ligated, which can keep DNA2 on the electrode surface during the denaturation. Amino group modified at the 5′ end of DNA2 captures AgNPs on the electrode surface, which provide sharp stripping peaks for microRNA quantification. This electrochemical approach offers a simple and sensitive platform for the detection of microRNA, which shows great utility in biomedical research and clinical diagnosis. |
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