Determination of metallothionein in Daphnia magna by modified square wave cathodic stripping voltammetry |
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| Institution: | 1. Department of Applied Chemistry, School of Science, Xi''an Jiaotong University, Xi''an, No. 28, Xianning West Road, 710049, PR China;2. School of Pharmacy, Health Science Center, Xi''an Jiaotong University, Xi''an, No. 76, Yanta West Road, 710061, PR China;1. Center for Developmental Neuropsychiatry, Department of Psychiatry, Columbia University & New York State Psychiatric Institute, New York, NY 10032, USA;2. Key laboratory of Brain Functional Genomics (MOE & STCSM), Institute of Cognitive Neuroscience, School of Psychology and Cognitive Science, East China Normal University, Shanghai, 20062, China;3. MRI Unit, Department of Psychiatry, Columbia University & New York State Psychiatric Institute, New York, NY 10032, USA;4. Columbia Center for Children''s Environmental Health, Mailman School of Public Health, Columbia University, New York, NY 10032, USA |
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| Abstract: | Metallothioneins (MTs) are widely used as biomarkers in environmental studies. However, eliminating interfering factors for MT determination in vivo by electrochemical detection is difficult. In this study, NO3− and Cl− were found to negatively affect MT determination in Daphnia magna by square wave cathodic stripping voltammetry (SWCSV). The relevant mechanism was analyzed, and results were used to modify the original electrochemical testing parameters and environmental conditions. This modified SWCSV achieved accurate MT concentrations in D. magna, leading to detection limits as low as picomolar levels. |
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