Separation of polypeptide antibiotics by reversed-phase high-performance liquid chromatography |
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Authors: | H -P Fiedler T Hörner A Wörn |
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Institution: | 1. Institut für Biologie 11, Lehrstuhl Mikrobiologie 1, Universit?t Tübingen, Auf der Morgenstelle 28, D-7400, Tübingen, FRG
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Abstract: | Summary The influence of different reversed-phase packings and the addition of acidic modifiers to the mobile phase was observed on
the separation of basic and neutral polypeptide antibiotics by gradient elution. A dependence of pore size, coverage, reaction
type and endcapping of the packings was not observed. Nevertheless, not all reversed-phase packings were suitable for the
separation of polypeptides, especially of basic molecules. The addition of phosphoric or perchloric acid to the mobile phase
prevented adsorption of the basic polypeptide antibiotics on the stationary phase. |
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Keywords: | Column liquid chromatography Adsorption effects Polypeptide antibiotics Quantitative determination |
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