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Fabrication of a novel poly(dimethylsiloxane) microchips with two sharpened stretching tips
作者姓名:Chun Ye Liu  Xu Xu  Hong Jun Gao  Jie Rong Chen School of Life Science and Technology  Xi'an Jiaotong University  Xi'an  China Shanghai Institute of Organic Chemistry  Chinese Academy of Sciences  Shanghai  China
作者单位:Chun Ye Liu,Xu Xu,Hong Jun Gao,Jie Rong Chen School of Life Science and Technology,Xi'an Jiaotong University,Xi'an 710049,China Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Shanghai 200032,China
摘    要:An integrated poly(dimethylsiloxane) (PDMS) microchip with two sharpened stretching has been presented. The sample was directly introduced into the separation channel through the stretching inlet tip without complicated power switching supplies and without injection cross-channel. Operations of running buffer refreshing or channel cleaning also becomes simple by vacuumed in one end and placed another tip into solution vial. The fabrication method can be easily applied in most analytical laboratories at low cost in the absence of soft lithography and plasma bonding equipments. Characteristics of the chips were tested and it can be used to separate fluorescence labeled molecules.

收稿时间:7 September 2006

Fabrication of a novel poly(dimethylsiloxane) microchips with two sharpened stretching tips
Chun Ye Liu,Xu Xu,Hong Jun Gao,Jie Rong Chen School of Life Science and Technology,Xi''''an Jiaotong University,Xi''''an ,China Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Shanghai ,China.Fabrication of a novel poly(dimethylsiloxane) microchips with two sharpened stretching tips[J].Chinese Chemical Letters,2007,18(2):221-224.
Authors:Chun Ye Liu  Xu Xu  Hong Jun Gao  Jie Rong Chen
Institution:1. School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China;2. Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China
Abstract:An integrated poly(dimethylsiloxane) (PDMS) microchip with two sharpened stretching has been presented. The sample was directly introduced into the separation channel through the stretching inlet tip without complicated power switching supplies and without injection cross-channel. Operations of running buffer refreshing or channel cleaning also becomes simple by vacuumed in one end and placed another tip into solution vial. The fabrication method can be easily applied in most analytical laboratories at low cost in the absence of soft lithography and plasma bonding equipments. Characteristics of the chips were tested and it can be used to separate fluorescence labeled molecules.
Keywords:Poly(dimethylsiloxane)  Microchip  Electrophoresis  Sample injection
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