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Unusual change in molecular weight of polyhydroxyalkanoate (PHA) during cultivation of PHA-accumulating Escherichia coli |
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| Authors: | Jumiarti Agus Manami Hyakutake Hideki Abe Yasuharu Satoh |
| Institution: | a Department of Innovative and Engineered Materials, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan b Asian Center for Environmental Research, Meisei University, 2-1-1 Hodokubo, Hino-shi, Tokyo 191-8506, Japan c Bioplastic Research Team, RIKEN Biomass Engineering Program, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan d Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo 060-8628, Japan |
| Abstract: | This study aimed to investigate the factors affecting molecular weight of poly(R)-3-hydroxybutyrate] P(3HB)] when polyhydroxyalkanoate (PHA) synthase (PhaRCBsp) from Bacillus sp. INT005 was used for P(3HB) synthesis in Escherichia coli JM109. It was found that the molecular weight of P(3HB) decreased with time in mid- and late-phase of culture and was strongly affected by culture temperature. At 37 °C culture temperature, the molecular weight of P(3HB) rapidly decreased from 4.4 × 105 to 4.8 × 104 with culture time, whereas it was almost unchanged at 25 °C. Kinetic analysis suggested that the decrease in molecular weight of P(3HB) was due to random scission of the polymer chain. The decrease in molecular weight of P(3HB) was not observed when PHA synthases other than PhaRCBsp were expressed. This study sheds light on the unique behaviour in molecular weight change of P(3HB) that is synthesized by E. coli expressing PhaRCBsp. |
| Keywords: | Polyhydroxyalkanoate PHA synthase Bacillus Molecular weight Random scission |
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