Affinity chromatography on vancomycin coupled to reversed phase liquid chromatography/ electrospray-ion trap mass spectrometry for the screening of combinatorial libraries |
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Authors: | F. Lynen F. Borremans P. Sandra |
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Affiliation: | (1) Department of Organic Chemistry, University of Gent, Krijgslaan 281 S4, 9000 Gent, Belgium |
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Abstract: | Summary The combination affinity chromatography-mass spectrometry can be drastically improved by introducing a reversed phase column prior to the mass spectrometric detection. The interactions of the macrocyclic antibiotic vancomycin with oligopeptides were used to illustrate the performance of the technique. A library of 36 peptides was successfully screened and the active compounds identified by electrospray MS(n). The strong affinity of compounds ending with (D)-alanine and with (D)-alanine or an aromatic (D)-amino acid in the penultimate position with vancomycin was confirmed. |
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Keywords: | Column liquid chromatography Affinity chromatography Vancomycin Mass spectroscopy detection Combinatorial chemistry Library screening |
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