Surfactin production from potato process effluent by Bacillus subtilis in a chemostat |
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Authors: | Karl S Noah Debby F Bruhn Gregory A Bala |
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Institution: | (1) Biotechnology Department, Idaho National Engineering and Environmental Laboratory, PO Box 1625, 83415-2203 Idaho Falls, ID |
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Abstract: | The biosurfactant surfactin has potential to aid in the recovery of energy resources (oil recovery) or subsurface organic
contaminants (environmental remediation). However, high medium and purification costs limit its use in these high-volume applications.
In previous work, we showed that surfactin could be produced from an inexpensive low-solids potato process effluent with minimal
amendments or pretreatments. Previous research has also shown that surfactin can be both produced in Bacillus subtilis cultures and recovered by foam fractionation in an airlift reactor. Results using both purified potato starch and unamended
low-solids potato process effluent as substrates for surfactin production indicate that the process is oxygen limited and
that recalcitrant indigenous bacteria in the potato process effluent hamper continuous surfactin production. The research
reported here features the use of a chemostat operated in batch mode for producing surfactin with concomitant use of antifoam
to prevent surfactant loss. The antifoam did not interfere with surfactin recovery by acid precipitation or its efficacy.
Initial trials took about 48 h to produce 0.9 g/L of surfactin from potato process effluent. Increasing the oxygen mass transfer
by increasing the stirring speed and adding a baffle decreased production time to 12–24 h and produced about 0.6 g/L of surfactin
from two different potato-processing facilities. |
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Keywords: | Bacillus subtilis biosurfactant surfactin alternate feedstock enhanced oil recovery |
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