Short polystyrene monolith-fritted micro-liquid chromatography columns for rapid isocratic analysis of pharmaceuticals direct from plasma |
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| Authors: | Cristina Legido-Quigley Norman W Smith |
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| Institution: | (1) Microseparations Group, Department of Pharmacy, King’s College London, Franklin-Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK;(2) Centre for Analytical Sciences, Department of Chemistry, Imperial College London, Exhibition Rd, London, SW7 2AY, UK |
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| Abstract: | The manufacture of micro-HPLC columns with combined stationary phases, a body of 3.5-μm XTerra-C18 particles, and poly(styrene–divinylbenzene) (PS–DVB) frits is described in detail. The efficiency of the columns was assessed by rapid separation of neutral and acid compound mixtures. Direct analysis of some pharmaceuticals in plasma resulted in lower limits of detection (LOD) for salmeterol xinofate of 12.5 nanograms on-column. |
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| Keywords: | Monolith frit Stationary phases MicroLC |
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