| 莱克多巴胺荧光胶乳颗粒免疫层析检测法的建立 |
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| 引用本文： | 崔浩,陈耀强,唐勇,向军俭,徐霞玲,颜露. 莱克多巴胺荧光胶乳颗粒免疫层析检测法的建立[J]. 分析测试学报, 2011, 30(7): 764-768. DOI: 10.3969/j.issn.1004-4957.2011.07.010 |
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| 作者姓名： | 崔浩 陈耀强 唐勇 向军俭 徐霞玲 颜露 |
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| 作者单位： | 暨南大学抗体工程研究中心,广东广州,510632 |
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| 基金项目： | 广东省部“产学研”资助项目（2009B090300452） |
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| 摘 要： | 基于免疫竞争层析法原理,建立了莱克多巴胺(RAC)的荧光胶乳颗粒免疫层析快速检测技术,用于猪肉组织中莱克多巴胺残留的检测.应用自制的抗RAC单抗,标记己胶乳荧光颗粒,将标记好的复合物喷涂于结合垫上.羊抗鼠IgG与人工合成的RAC - BSA检测抗原包被在NC膜表面,分别作为质控线(C线)与检测线(T线).检测过程中RA...
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| 关 键 词： | 莱克多巴胺 胶乳荧光颗粒 免疫层析(ICA) 猪肉 |
Development of an Immunochromatography Assay with Fluorescent Latex Particles for the Rapid Detection of Ractopamine |
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| CUIHao,CHENYao-qiang,TANGYong,XIANGJun-jian,XUXia-ling,YANLu. Development of an Immunochromatography Assay with Fluorescent Latex Particles for the Rapid Detection of Ractopamine[J]. Journal of Instrumental Analysis, 2011, 30(7): 764-768. DOI: 10.3969/j.issn.1004-4957.2011.07.010 |
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| Authors: | CUIHao CHENYao-qiang TANGYong XIANGJun-jian XUXia-ling YANLu |
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| Affiliation: | CUI Hao,CHEN Yao-qiang,TANG Yong,XIANG Jun-jian,XU Xia-ling,YAN Lu(Antibody Engineering Center,Jinan University,Guangzhou 510632,China) |
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| Abstract: | A fluorescent latex immunochromatographic assay for the detection of ractopamine residues in pork was established based on competition principle.Fluorescent latex particles were marked with anti-RAC monoclonal antibody(McAb),the conjugate was sprayed onto the conjugate pad.Then goat anti-mice IgG and the artificial antigen RAC-BSA were prepared and jet-positioned onto nitrocellulose(NC) membrane as the control line(C) and the test line(T),respectively.Sample pad,conjugate pad,nitrocellulose membrane and abs... |
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| Keywords: | ractopamine fluorescent latex particles immunochromatography assay(ICA) pork |
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