| 荧光光谱法研究青蒿素类抗疟药与CYP2B6酶的相互作用 |
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| 引用本文： | 孙坤,张爱平,王锐利,李赟,张立伟,张淑秋. 荧光光谱法研究青蒿素类抗疟药与CYP2B6酶的相互作用[J]. 分析测试学报, 2011, 30(7): 750-754. DOI: 10.3969/j.issn.1004-4957.2011.07.007 |
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| 作者姓名： | 孙坤 张爱平 王锐利 李赟 张立伟 张淑秋 |
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| 作者单位： | 1. 山西医科大学药学院,山西太原,030001
2. 山西大学分子科学研究所,山西太原,030001 |
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| 基金项目： | 国家自然科学基金资助项目（30572367,30973589）；山西省回国留学人员科研项目（2009-47）；山西省医科大学青年基金资助项目（02200704） |
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| 摘 要： | 在模拟人体生理条件下,采用荧光光谱法及同步荧光法研究了抗疟药青蒿素(ART)和双氢青蒿素(DHA)与药物代谢酶CYP2B6之间的相互作用.结果表明,在温度为296～303 K时,ART和DHA对CYP2B6的猝灭机制均以静态猝灭为主,当温度由303 K升高至310 K时,则为静态猝灭和动态猝灭共同作用的混合猝灭机制；在...
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| 关 键 词： | 荧光光谱法 同步荧光光谱法 青蒿素 双氢青蒿素 CYP2B6 |
Interactions between Artemisinin or Dihydroartemisinin and CYP2B6 by Fluorescence Spectroscopy |
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| SUNKun,ZHANGAi-ping,WANGRui-li,LIYun,ZHANGLi-wei,ZHANGShu-qiu. Interactions between Artemisinin or Dihydroartemisinin and CYP2B6 by Fluorescence Spectroscopy[J]. Journal of Instrumental Analysis, 2011, 30(7): 750-754. DOI: 10.3969/j.issn.1004-4957.2011.07.007 |
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| Authors: | SUNKun ZHANGAi-ping WANGRui-li LIYun ZHANGLi-wei ZHANGShu-qiu |
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| Affiliation: | SUN Kun1,ZHANG Ai-ping1,WANG Rui-li1,LI Yun1,ZHANG Li-wei2,ZHANG Shu-qiu1(1.Department of Pharmacy,Shanxi Medical University,Taiyuan 030001,China,2.Institute of Molecular Science,Shanxi University,China) |
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| Abstract: | The binding reactions between antimalarial agent,e.g.artemisinin(ART) or dihydroartemisinin(DHA),and drug-metabolizing enzyme CYP2B6 were studied by fluorescence quenching spectroscopy at simulated physiological conditions.The structural information of CYP2B6 during their interactions was investigated by synchronous fluorometric method.The results showed that,for both reactions of ART with CYP2B6 and DHA with CYP2B6,the main quenching mechanism was static quenching at temperature range of 296-303 K,and turn... |
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| Keywords: | fluorescence spectroscopy synchronous fluorescence spectroscopy artemisinin dihydroartemisinin CYP2B6 |
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