Bovine serum albumin adsorption on nano-rough platinum surfaces studied by QCM-D |
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Authors: | Dolatshahi-Pirouz A Rechendorff K Hovgaard M B Foss M Chevallier J Besenbacher F |
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Affiliation: | Department of Physics and Astronomy, University of Aarhus, DK-8000 Aarhus C, Denmark. |
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Abstract: | The adsorption of bovine serum albumin (BSA) on platinum surfaces with a root-mean-square roughness ranging from 1.49nm to 4.62nm was investigated using quartz crystal microbalance with dissipation (QCM-D). Two different BSA concentrations, 50microg/ml and 1mg/ml, were used, and the adsorption studies were complemented by monitoring the antibody interaction with the adsorbed BSA layer. The adsorption process was significantly influenced by the surface nano-roughness, and it was observed that the surface mass density of the adsorbed BSA layer is enhanced in a non-trivial way with the surface roughness. From a close examination of the energy dissipation vs. frequency shift plot obtained by the QCM-D technique, it was additionally observed that the BSA adsorption on the roughest surface is subject to several distinct adsorption phases revealing the presence of structural changes facilitated by the nano-rough surface morphology during the adsorption process. These changes were in particular noticeable for the adsorption at the low (50microg/ml) BSA concentration. The results confirm that the nano-rough surface morphology has a significant influence on both the BSA mass uptake and the functionality of the resulting protein layer. |
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Keywords: | BSA Nano-topography QCM-D Platinum Protein adsorption |
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