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In vitro photodynamic inactivation of Candida spp. growth and adhesion to buccal epithelial cells |
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| Authors: | Betânia Maria Soares Danielle Letícia da Silva Gerdal Roberto Sousa José Cláudio Faria Amorim Maria Aparecida de Resende Marcos Pinotti Patrícia Silva Cisalpino |
| Institution: | 1. Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, UFMG, Av. Antônio Carlos, 6627 – CEP.: 31270-901, Belo Horizonte, Minas Gerais, Brazil;2. Department of Mechanical Engineering, Bioengineering Laboratory, Federal University of Minas Gerais, UFMG, Belo Horizonte, Minas Gerais, Brazil |
| Abstract: | In this study, photodynamic inactivation (PDI) was used to inhibit in vitro growth and adhesion of different Candida isolates to buccal epithelial cells (BEC). Experimental conditions were optimized and 25 μM toluidine blue O (TBO) and 15 min of irradiation time by light emitting diode (LED) (energy density of 180 J/cm2) were selected due to higher reductions in cellular viability obtained after treatment. Reduction media of Log10 3.41 in viable cellular growth and media of 55% in the inhibition of adhesion to buccal epithelial cells were obtained. Two fluconazole resistant isolates were susceptible to PDI (Log10 3.54 in IB05 and Log10 1.95 in CG09) and a second session of this treatment for CG09 isolate inhibited cellular viability in 100%, without producing heat. The results permit to conclude that photodynamic inactivation under these experimental conditions would be a possible alternative approach to inhibit Candida spp. cellular growth and adhesion to buccal epithelial cells. |
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