| 人体尿液中青霉素G代谢物的确认 |
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| 引用本文： | 陈聪,严慧,沈保华,卓先义.人体尿液中青霉素G代谢物的确认[J].化学通报,2012(2):144-149. |
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| 作者姓名： | 陈聪 严慧 沈保华 卓先义 |
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| 作者单位： | 复旦大学上海医学院法医系;司法部司法鉴定科学技术研究所上海市法医学重点实验室 |
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| 摘 要： | 建立了利用电场轨道阱回旋共振组合质谱仪(LTQ-Orbitrap)检测人体尿样中青霉素G及其代谢产物的分析方法。目标化合物分离鉴定选用PLRP-S聚合色谱柱,以含0.1%(体积比)甲酸溶液-乙腈为流动相,梯度洗脱分离后,选用Data-dependent MSn模式进行全扫描分析。尿样中首次检出青霉素甲酰化代谢产物(m/z 381)。青霉素新代谢物的发现对食品安全及法医毒物分析具有重要意义。
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| 关 键 词： | 青霉素G 代谢物 尿液 静电场轨道阱回旋共振组合质谱仪 |
Confirmation of Penicillin G Metabolites in Human Urine |
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| Chen Cong,Yan Hui,Shen Baohua,Zhuo Xianyi.Confirmation of Penicillin G Metabolites in Human Urine[J].Chemistry,2012(2):144-149. |
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| Authors: | Chen Cong Yan Hui Shen Baohua Zhuo Xianyi |
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| Institution: | 1Forensic Medicine Department of Shanghai Medical College of Fudan University,Shanghai 200032;2Shanghai Key Laboratory of Forensic Medicine,Institute of Forensic Science,Ministry of Justice,Shanghai 200063) |
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| Abstract: | A method for confirmation of penicillin G and its metabolites in human urine using LC-LTQ-Orbitrap was developed.Urine was directly deproteinated by acetonitrile,the target compounds in urine samples were separated by PLRP-S column with the solution of 0.1% formic acid aqueous in acetonitrile,finally analyzed with data-dependent MSn full scan mode.A new compound(m/z 381) was detected in human urine.These results could be applied to detection of drug residues in the field of food safety and analytical toxicology. |
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| Keywords: | Penicillin G Metabolites Urine LTQ-Orbitrap |
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