| Crystallization and Preliminary Crystallographic Analysis of β- Trichosanthin, an Isoform of Trichosanthin from the Root Tuber of Trichosanthe kirilowii |
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| 作者姓名： | HOU Xiao-Min CHEN Ming-Huang PENG Qi CHEN Li-Qing Meehan Edward J HUANG Ming-Dong |
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| 作者单位： | State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter，The Chinese Academy of Sciences，State Key Laboratory of Structural Chemistry，Fujian Institute of Research on the Structure of Matter，The Chinese Academy of Sciences，State Key Laboratory of Structural Chemistry，Fujian Institute of Research on the Structure of Matter，The Chinese Academy of Sciences，Laboratory for Structural Biology，Department of Chemistry，Graduate Programs of Biotechnology，Chemistry and Materials Science，University of Alabama in Huntsville，Laboratory for Structural Biology，Department of Chemistry，Graduate Programs of Biotechnology，Chemistry and Materials Science，University of Alabama in Huntsville，State Key Laboratory of Structural Chemistry，Fujian Institute of Research on the Structure of Matter，The Chinese Academy of Sciences，Fuzhou，Fujian 350002，China Graduate School of the Chinese Academy of Sciences，Beijing 100039，China，Fuzhou，Fujian 350002，China，Fuzhou，Fujian 350002，China，Huntsville，AL 35899，USA，Huntsville，AL 35899，USA，Fuzhou，Fujian 350002，China |
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| 基金项目： | the NNSF of China (No.39970872,No.30772587)，the Natural Science Foundation of Fujian Province (C97052,C0510012)，Special Fund of Fujian Development and Reform Commission，the National Science Foundation of USA |
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| 摘 要： | β-Trichosanthin,a type 1 ribosome-inactivating protein (RIP) isolated from the root tuber of Trichosanthe kirilowii Maxim,is an isoform of trichosanthin. Here we report its crystal-lization in two crystal forms using the hanging-drop vapor-diffusion method. The form A and form B crystals belong to the orthorhombic space group P212121 and monoclinic space group P21,respectively. X-ray data have been collected to 1.6 and 1.2 resolution for form A and form B crystals,respectively,using a synchrotron source.
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| 关 键 词： | 核蛋白体 天花粉蛋白 结晶 异构重整产物 |
| 收稿时间： | 2007-01-22 |
| 修稿时间： | 2007-03-15 |
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